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KMID : 0903519850280010013
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 1 p.13 ~ p.18
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Isolation of Uracil and Tryptophan Auxotrophs of Bacillus subtilis and Regeneration of their Protoplasts
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Abstract
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To investigate the fusion of bacterial protoplasts, two auxotrophic mutants of Bacillus subtilis were isolated after treatment with nitrosoguanidine. Two auxotrophs were uracil-auxotroph and tryptophan-auxotroph. Back mutation frequencies of ura- and trp- -auxotrophs were 2.4×10^(-8) and less than 1.0×10^(-8) respectively. The optimal pH and temperatnre of protoplast formation with lysozyme were 6.5 and 30℃. The optimal lysozyme concentration was 200㎍/㎖. The regeneration frequency of protoplast was 3.3%.
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